Description
Intended Uses: The human sVCAM-1/CD106 ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of soluble Vascular Cellular Adhesion Molecule-1 (sVCAM-1) in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human sVCAM-1.
Principle of the Assay: The sVCAM-1 Kit is a solid phase sandwich Enzyme Linked Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for sVCAM-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known sVCAM-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the sVCAM-1 antigen and a biotinylated monoclonal antibody specific for sVCAM-1 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sVCAM-1 present in the samples